| 货号 | AF5558-SP |
| 别名 | 150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; DKFZp686N08236; FLJ97572; glucose-regulated protein 170; Grp170; GRP-170; HSP12A; HYOU1; hypoxia up-regulated 1; hypoxia up-regulated protein 1; ORP-150; ORP150FLJ94899; oxygen regulated protein (150kD) | 全称 | Oxygen Regulated Protein, 150 kDa |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ORP150/HSP12A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) HSP-70, rhHSPA-2, and rhHSPH-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10525 (Human); 12282 (Mouse); 192235 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human ORP150/HSP12A Met695-Leu994 Accession # Q9Y4L1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ORP150 (Oxygen-related protein of 150 kDa) is a member of the heat shock protein 70 family of molecules. Although the predicted MW is 111 kDa, it runs at 150 kDa in SDS-PAGE. The discrepancy may not be anomalous, since it is secreted into the ER lumen and likely to be glycosylated and/or phosphorylated. ORP150 is highly expressed in cells involved in secretion such as pancreatic acinar cells and hepatocytes. Mature human ORP150 is 967 amino acids (aa) in length. It contains two HSP70 signatures (aa 230‑243 and 380‑394) and an ER-retention signal (aa 996‑999). There are multiple isoforms. Three show alternate start sites at Met88, Met152, and a Met 44 aa upstream of the standard start site. Four isoforms also show deletions of aa 148‑958, 51‑59, 603‑664 and 164‑507, respectively. There is one 44 aa substitution for aa 603‑999. Over aa 1‑994, human ORP150 shares 92% aa identity with mouse ORP150. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ORP150/HSP12A by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human breast cancer tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ORP150/HSP12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ORP150/HSP12A at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
