| 货号 | AF5557-SP |
| 别名 | MGC3467; prolactin regulatory binding-element protein; prolactin regulatory element binding; prolactin regulatory element-binding protein; SEC12; SEC12Mammalian guanine nucleotide exchange factor mSec12 | 全称 | Prolactin Regulatory Element Binding |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PREB in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10113 (Human); 50907 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The ER-Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PREB Met1-Ser388 Accession # Q9HCU5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PREB (Prolactin regulatory element-binding protein; also mSEC12) is a 45 kDa member of the WD-repeat protein family. Although it is ubiquitously expressed, its expression level varies, tissue-to-tissue. It apparently acts as both a DNA-binding transcription factor, and a catalyst for vesicle formation from the ER. Human PREB is a 417 amino acid (aa) type II transmembrane protein. It is found in the ER and nuclear envelope, and may undergo splicing to generate a transcriptional factor. PREB contains a large cytoplasmic region (aa 1‑388) that displays three WD repeats (aa 152‑337), and an eight aa lumenal domain (aa 410‑417). The transcription factor region lies between aa 175‑417. Multiple splice forms exist. There is an alternate start site at Met73 that may be accompanied by either a 47 aa substitution for aa 388‑417, or a 14 aa substitution for aa 409‑417. Over aa 1‑388, human PREB shares 88% aa identity with mouse PREB. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human PREB by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti‑Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
