| 货号 | AF5555-SP |
| 别名 | Major synaptic vesicle protein p38; MRX; MRXSYP; synaptophysin; SYP |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Synaptophysin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6855 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Synaptophysin Glu50-Phe106 Accession # P08247 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Synaptophysin in Human Brainstem Tissue. Synaptophysin was detected in immersion fixed paraffin-embedded sections of human brainstem tissue using Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olivary nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. *Not recommended for Immunohistochemistry/Immunocytochemistry on mouse and rat samples. | |
Detection of Human, Mouse, and Rat Synaptophysin by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Synaptophysin at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
