| 货号 | AF5546-SP |
| 别名 | Aryl sulfotransferase 1; EC 2.8.2; EC 2.8.2.1; HAST1/HAST2; Phenol sulfotransferase 1; Phenol-sulfating phenol sulfotransferase 1; P-PST 1; P-PST; PST; ST1A1; ST1A3; STP1 ; STP1MGC5163; STPMGC131921; sulfotransferase 1A1; sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1; SULT1A1; Thermostable phenol sulfotransferase; thermostable phenol sulfotransferase1; ts-PST; TSPST1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SULT1E1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6817 (Human); 20887 (Mouse); 83783 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Contribution of nutritional deficit to the pathogenesis of the nonthyroidal illness syndrome in critical illness: a rabbit model study. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1 Glu2-Leu295 Accession # P50225 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity (2). The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters (3). Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers (2). The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
