Human SULT1E1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human SULT1E1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF5545-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5545-SP
别名	EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC 2.8.2.4; SULT1E1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhSulfotransferase 2A1 and rhSulfotransferase 4A1 is observed.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6783 (Human); 20860 (Mouse); 25355 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1 Asn2-Ile294 Accession # P49888
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Gong, H. et al. (2008) Cancer Res. 68:7386. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529. Miksits, M. et al. 2005, Xenobiotica 35:1101. Takase, Y. et al. (2007) The Prostate 67:405. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613. Wu, Z.L., et al. (2002) Faseb J. 16:539.

参考图片

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.