| 货号 | AF5544-SP |
| 别名 | Golgi-Localized Protein; Golgi-Localized Syntaphilin-Related Protein; GOLSYN A Protein; GOLSYN B Protein; GOLSYN C Protein; GOLSYN; Implicated In Syntaxin Trafficking In Neurons; KIAA1472; Microtubule-Associated Protein; OCSYN; SNPHL; SYBU; Syntabulin (Syntaxin-Interacting); syntabulin; syntaphilin-like; Syntaxin-1-Binding Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Syntabulin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55638 (Human); 319613 (Mouse); 500865 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntabulin Gln489-Arg656 Accession # Q9NX95 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntabulin (also Golgi-localized synaphilin-related protein) is a 70-75 kDa molecule that belongs to the syntaxin-binding and kinesin-1 family of motor adaptor proteins. It is highly spliced, with various forms associated with different tissues. In neurons, it anchors syntaxin-1 to KIF5B, creating a complex by which synaptic components can be transported to synaptic terminals. Human Syntabulin is a type II transmembrane (TM) protein 663 amino acids (aa) in length. It contains one coiled-coil region (aa 271‑353) and a TM domain between aa 606‑626. Alternate splice forms show alternate start sites at Met120 and Met207, a five aa substitution for aa 1‑8, a 16 aa insertion after Lys8, and an eight aa substitution for aa 1‑76. Over aa 486‑653, human Syntabulin shares 83% aa identity with mouse Syntabulin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Syntabulin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntabulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5544) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntabulin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
