| 货号 | AF5530-SP |
| 别名 | EC 2.7.11.1; FLJ36101; H_RG152G17.1a; H_RG152G17.1b; serine kinase SRPK2; Serine/arginine-rich protein-specific kinase 2; serine/arginine-rich splicing factor kinase 2; serine/threonine-protein kinase SRPK2; SFRS protein kinase 2SFRSK2; SFRSK2; SR protein kinase 2; SR-protein-specific kinase 2; SRSF protein kinase 2; WUGSC:H_RG152G17.1a | 全称 | SFRS Protein Kinase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human SRPK2 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6733 (Human); 20817 (Mouse); 296753 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SRPK2 Asn313-Ala466 Accession # P78362 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SRPK2 (Ser/Arg-richprotein specific kinase2) is a cytoplasmic, 115‑120 kDa member of the CMGC Ser/Thr protein kinase family of enzymes. It is expressed in many experimental cell lines and serves to selectively phosphorylate Ser on RS domain-containing proteins. This phosphorylation activates RS domain-containing non-snRNP proteins in the spliceosome complex, and initiates mRNA splicing. Human SRPK2 is 688 amino acids (aa) in length. It contains a Pro-rich region (aa 21‑45) that may interact with WW domain-containing proteins, and a split kinase domain (aa 79‑226 and 524‑646). Phosphorylation on S52 and S588 regulates activity. There are at least two splice variants. One shows a 24 aa substitution for aa 1‑13, while a second shows a nine aa substitution for aa 538‑688. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SRPK2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human SRPK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SRPK2 at approximately 115 to 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
