| 货号 | AF5528-SP |
| 别名 | beta-synuclein; SNCB; synuclein, beta |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Synuclein-beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Synuclein-alpha and less than 1% cross-reactivity with rhSynuclein-gamma is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6620 (Human); 104069 (Mouse); 113893 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Synuclein-beta Gly47-Glu120 Accession # Q16143 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Synuclein-beta is a 19 kDa, 134 amino acid (aa) cytoplasmic phosphoprotein that is a member of a family of small, highly conserved proteins, including synucleins alpha, beta, and gamma (synoretin), localized predominantly in presynaptic nerve terminals in the brain. Synuclein-beta associates with Synuclein-alpha, preventing it from aggregating. Abnormal Synuclein-alpha aggregates form filamentous inclusions (Lewy bodies) in neurodegenerative diseases including Parkinsons disease. Within the region used as an immunogen, human SNCB shares 99% aa identity with mouse and rat Synuclein-beta. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Synuclein-beta in Human Brain. Synuclein‑ beta was detected in immersion fixed paraffin-embedded sections of human brain (globus pallidus) using Sheep Anti-Human Synuclein‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the presynaptic terminals. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Synuclein-beta by Western Blot. Western blot shows lysates of human brain cortex, Alzheimer grey matter and white matter, and human cerebellum, brain cortex, and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Synuclein‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Synuclein-beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
