Human IRF4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IRF4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF5525-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5525-SP
别名	interferon regulatory factor 4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5
全称	Interferon Regulatory Factor 4
反应种属	Human
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IRF4 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IRF3, rhIRF5, and rhIRF6 is observed.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3662 (Human); 16364 (Mouse); 291100 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activin-A co-opts IRF4 and AhR signaling to induce human regulatory T cells that restrain asthmatic responses Authors: S Tousa, M Semitekolo, I Morianos, A Banos, AI Trochoutso, TM Brodie, N Poulos, K Samitas, M Kapasa, D Konstantop, G Paraskevop, M Gaga, CM Hawrylowic, F Sallusto, G Xanthou Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IRF4 Glu130-Glu451 Accession # Q15306
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Interferon regulatory factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Markers Conventional/Classical Dendritic Cells Dendritic Cell Development ICC / IHC Images: IRF4 Interferon-Regulatory Factor Transcription Factors Macrophage Activation Markers Th2 Cells Th9 Cells

参考图片

Detection of Human IRF4 by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line, Raji human Burkitts lymphoma cell line, and RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human IRF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5525) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRF4 in Human Tonsil. IRF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human IRF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5525) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.