| 货号 | AF5510-SP |
| 别名 | DDIT-1; DDIT1growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; GADD45a; GADD45DNA-damage-inducible transcript 1; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible, alpha | 全称 | Growth Arrest and DNA Damage Gene alpha |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human GADD45 alpha in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1647 (Human); 13197 (Mouse); 25112 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GADD45 alpha Met1-Arg165 Accession # P24522 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GADD45 alpha by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45 alpha. PVDF membrane was probed with 1 µg/mL Human GADD45 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for GADD45 alpha was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
