| 货号 | AF5509-SP |
| 别名 | FLJ34027; GAP-associated tyrosine phosphoprotein p62; KH domain containing, RNA binding, signal transduction associated 1; KH domain-containing, RNA-binding, signal transduction-associated protein 1; KHDRBS1; p21 Ras GTPase-activating protein-associated p62; p62; p68; SAM68; Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68); Src-associated in mitosis 68 kDa protein | 全称 | Src-associated in Mitosis 68 kDa Protein |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse SAM68 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10657 (Human); 20218 (Mouse); 117268 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human and mouse SAM68 Met108-Pro280 Accession # Q07666 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SAM68 (Src associated in mitosis 68 kDa; also KH domain-containing, RNA-binding, signal transduction-associated protein 1) is a 62‑68 kDa member of the KHDRBS family of proteins. It is associated with multiple receptor systems (i.e.- TCR and leptin), as an adaptor protein by its SH2 and SH3 domains, and binds to UAAA motifs in mRNA. Human SAM68 is 443 amino acids (aa) in length. It contains one SGQ region (aa 96‑157) and one KH domain (aa 171‑197). Post‑translational modification of SAM68 alters its partnership with other molecules. For example, methylation on ArgGly motifs reduce RNA binding, acetylation on multiple Lys residues increases RNA binding, and phosphorylation on Tyr promotes SH3 domain interaction. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse SAM68 by Western Blot. Western blot shows nuclear extracts of Raji human Burkitts lymphoma cell line, Daudi human Burkitts lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse SAM68 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5509) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SAM68 at approximately 60-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
