| 货号 | AF5480-SP |
| 别名 | alcadein gamma; Alcadein-gamma; alcagamma; ALC-GAMMA; cadherin-related family member 13; calsyntenin 2; calsyntenin-2; CDHR13; CLSTN2; CS2; CS2alcadein-gamma; CSTN2; FLJ39113; FLJ39499; MGC119560 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Calsyntenin-2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Calsyntenin-1 and rhCalsyntenin-3 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64084 (Human); 64085 (Mouse); 171394 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-2 Ser21-Thr834 (Val366Ile) Accession # Q9H4D0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Calsyntenin-2 (CS-2) is a 120-135 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi, and the plasma membranes of almost all neurons. It is a calcium-binding protein that interacts with APP and X11L. In this regard, it appears to regulate APP cleavage and gene activation, and likely impacts post-synaptic signaling. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes AICD gene activation. Mature human CS-2 is a 935 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 44-280) in its extracellular region (aa 21-831) and a 103 aa cytoplasmic tail. Proteolytic cleavage by ADAM10 occurs between His803 and Leu804 to generate a 105-110 kDa extracellular fragment. Additional processing of the transmembrane fragment by gamma -secretase creates a 3 kDa fragment (aa 804-834) plus a 25 kDa cytoplasmic protein. Potential isoform variants show a premature truncation after Gln608, and the use of an alternative start site at Met409. Over aa 21-834, human CS-2 shares 95% aa identity with mouse CS-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Calsyntenin‑2 in Human Brain. Calsyntenin‑2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Calsyntenin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5480) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
