| 货号 | AF5474-SP |
| 别名 | Apoptosis inducer NBK; BCL2-interacting killer (apoptosis-inducing); bcl-2-interacting killer; BIK; BIP1; BP4; NBK; NBKapoptosis-inducing NBK | 全称 | Bcl-2 Interacting Killer |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human BIK. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 638 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BIK Met1-Gln135 Accession # Q13323 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-2-interacting killer (BIK; also known as NBK, BP4 and BIP-1) is a 19 kDa member of the BH3-only subfamily, Bcl-2 family of proteins. It is widely expressed, and serves as an initiator of cell death. It apparently participates in apoptosis by sequestering antiapoptotic cytoplasmic proteins of the Bcl-2 family and in autophagy by regulating cytosolic calcium levels. Human BIK-1 is 160 amino acids (aa) in length. It contains one BH3 domain (aa 57‑74), a “death segment” (aa 121‑134) and a transmembrane domain (aa 136‑156). BIK is likely to embed in both outer mitochondrial and ER membranes. Over amino acids 1‑135, human BIK shares only 45% aa identity with mouse BIK. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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BIK in Human Lymphoma. BIK was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human BIK by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BIK at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
