| 货号 | AF5464-SP |
| 别名 | APG10 autophagy 10-like (S. cerevisiae); APG10; APG10L; APG10-like; ATG10 autophagy related 10 homolog (S. cerevisiae); Autophagy-related protein 10; DKFZp586I0418; EC 6.3.2.-; FLJ13954; PP12616; ubiquitin-like-conjugating enzyme ATG10 | 全称 | Autophagy Related 10 Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human ATG10 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 83734 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ATG10 Met1-Thr190 Accession # Q9H0Y0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ATG10 (autophagy-related protein 10; also APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates non‑covalently with an ATG16 multimer to generate an autophagosome. Human ATG10 is 220 amino acids in length and contains an active site at Cys166 that forms a thiol ester bond with the C-terminal Gly of ATG12. There are multiple isoform variants. One shows a deletion of aa 37‑72, a second contains an alternate start site at Met43 that is accompanied by a 39 aa substitution for aa 152‑220, and a third shows a 53 aa substitution for aa 73‑220. Over amino acids 1‑190, human ATG10 shares 79% aa identity with mouse ATG10. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ATG10 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5464) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
