| 货号 | AF5455-SP |
| 别名 | Cell migration-inducing gene 10 protein; EC 2.7.2.3; MGC142128; MGC8947; MIG10; PGKA; PGKAMGC117307; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP2 | 全称 | Phosphoglycerate Kinase-1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat PGK1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5230 (Human); 18655 (Mouse); 24644 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1 Met1-Ile417 Accession # P00558 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro‑B cell line, A20 mouse B cell lymphoma cell line, Rat‑2 rat embryonic fibroblast cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
