| 货号 | AF5448-SP |
| 别名 | CARB; CIP-1-associated regulator of cyclin B; STX8; syntaxin 8; syntaxin-8 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Syntaxin 8 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9482 (Human); 55943 (Mouse); 59074 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Syntaxin 8 Met1-Gly215 Accession # Q9UNK0 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Syntaxin 8 (STX8) is a 28 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in Golgi, endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX8 is involved in endosomal homotypic fusion events as well as protein trafficking. Human STX8 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 236 amino acids (aa) in length. It contains a coiled-coil region (aa 42‑65), a t-SNARE domain (aa 152‑207) that is likely involved in protein-protein interactions, and a short, four amino acid C-terminal luminal sequence. Over aa 1‑215, human STX8 shares 92% aa identity with mouse STX8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Syntaxin 8 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Syntaxin 8 by Western Blot. Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Syntaxin 8 was detected as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Syntaxin 8 in Human Brain. Syntaxin 8 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse/Rat Syntaxin 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5448) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
