| 货号 | AF5447-SP |
| 别名 | Charcot-Leyden crystal proteinLGALS10A; CLC; EC 3.1.1.5; eosinophil lysophospholipase; GAL10; Gal-10; galectin 10; galectin-10; LGALS10; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Galectin-10 in direct ELISAs and Western blots.In direct ELISAs, less than 1% cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -9 and -14 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1178 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Galectin-10, a potential biomarker of eosinophilic airway inflammation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Galectin‑10 Ser2-Arg142 Accession # Q05315 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one Galectin domain (aa 6‑138) that contains two dimerization motifs (aa 6‑10 and 131‑135). Two molecular weight isoforms of 15 and 14 kDa have been described that are not yet characterized. There is no known structural rodent counterpart to human Galectin-10. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Galectin‑10 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Goat Anti-Human Galectin‑10 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5447) followed by CFS-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) and Mouse Anti-Human CD25/IL-2 R alpha Allophycocyanin-conjugated Monoclonal Antibody (Catalog # FAB1020A).Quadrant markers were set based on control antibody staining (Catalog # AB‑108‑C). | |
Detection of Human Galectin‑10 by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
