| 货号 | AF5407-SP |
| 别名 | dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYRK1; MNBEC 2.7.110EC 2.7.12; MNBH | 全称 | Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 1A |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and rat DYRK1A in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1859 (Human); 13548 (Mouse); 25255 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Truncation and Activation of Dual Specificity Tyrosine Phosphorylation-regulated Kinase 1A by Calpain I: A MOLECULAR MECHANISM LINKED TO TAU PATHOLOGY IN ALZHEIMER DISEASE. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DYRK1A Arg438-Thr511 Accession # Q13627 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DYRK1A (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 1A; also hMNB) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK1A is ubiquitously expressed and shows dual substrate specificity. Autophosphorylation on Tyr321 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include APP, Tau, STAT3, and Cyclin L2. Human DYRK1A is 763 amino acids (aa) in length. It contains two NLSs (aa 69‑105 and 109-150), a kinase catalytic domain (aa 159-479), and C-terminal poly-Ser and poly-His regions. DYRK1A activity has been implicated in neuronal development and Down syndrome. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
DYRK1A in Human Kidney. DYRK1A was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat samples. | |
Detection of Human/Rat DYRK1A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DYRK1A at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human DYRK1A by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DYRK1A at approximately 113 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat DYRK1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5407) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
