| 货号 | AF5350-SP |
| 别名 | C10orf59; C10orf59chromosome 10 open reading frame 59; EC 1.4; FLJ11218; MAO-C; Monoamine oxidase-C; RENALASE; renalase, FAD-dependent amine oxidase; RNLS |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Renalase in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55328 (Human); 67795 (Mouse); 361751 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of a receptor for extracellular renalase. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Renalase Ala2-Ile342 Accession # NP_001026879 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Renalase is a novel, 35‑37 kDa FAD-dependent amine oxidase that is secreted primarily by glomerular and renal tubule epithelium. It presumably degrades catecholamines and lowers blood pressure by decreasing heart rate and cardiac contractility. In addition to renal cells, Renalase is produced by skeletal and cardiac muscle fibers. The human Renalase precursor is 342 amino acids (aa) in length. It contains an FAD binding domain (aa 4‑35) that overlaps a signal sequence (aa 1‑17), followed by an amine oxidase segment (aa 75‑339). Renalase circulates as both a monomer (from kidney) and homodimer (from heart). There is one potential splice variant that shows a 22 aa substitution for aa 294‑342. Over aa 2‑342, human Renalase shows 72% aa identity to mouse Renalase. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Renalase by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renalase at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
