| 货号 | AF5346-SP |
| 别名 | alcadein alpha 1; Alcadein-alpha; ALC-ALPHA; alcalpha1; alcalpha2; Alzheimer-related cadherin-like protein; cadherin-related family member 12; calsyntenin 1; calsyntenin-1; CDHR12; CLSTN1; CS1; CSTN1; FLJ32258; KIAA0911alcadein-alpha; Non-classical cadherin XB31alpha; non-classical cadherin XB31alpha1; PIK3CD; XB31alpha |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Calsyntenin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Calsyntenin-2 and rhCalsyntenin-3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22883 (Human); 65945 (Mouse); 313717 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-1 isoform 2 Arg30-Thr851 Accession # AAH33902 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Calsyntenin-1 (CST-1; also Alcadein-alpha ) is a 140‑150 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi and on the plasma membranes of almost all neurons. It is a calcium-binding protein that may regulate post-synaptic signaling and APP cleavage and gene activation. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes APP AICD gene activation. Mature human CST-1 is a 953 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 38‑265) in its extracellular region (aa 29‑859) and a 101 aa cytoplasmic tail. Extracellular cleavage occurs at Met825 Ala826, generating a soluble 115 kDa ECD. Isoforms involve a 78 aa substitution for aa 917‑981, plus deletions of aa 72‑81 and 507‑525. Over aa 30‑851, human CST-1 shares 92% aa identity with mouse CST-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Calsyntenin‑1 by Western Blot. Western blot shows lysates of human brain cortex and brain cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Calsyntenin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Calsyntenin‑1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
