Human Girdin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Girdin Affinity Purified Polyclonal Ab (25 UG)

货号： AF5345-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5345-SP
别名	Akt phosphorylation enhancer; APE; APEGIRDIN; CCDC88A; coiled-coil domain containing 88A; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; G{alpha}-interacting vesicle-associated protein; Girders of actin filament; girdin; GIV; GIVFLJ10392; GRDN; GRDNAKT-phosphorylation enhancer; HkRP1; HkRP1Galpha-interacting vesicle-associated protein; Hook-related protein 1; KIAA1212DKFZp686D0630
全称	Girders of Actin Filament
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects endogenous human Girdin in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	55704 (Human); 108686 (Mouse); 305605 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation. Authors: Tomiyama, L, Sezaki, T, Matsuo, M, Ueda, K, Kioka, N Oncogene, 2015;34(9):1141-9. Species: Human Sample Type: Cell Lysates Application: WB Akt-Girdin signaling in cancer-associated fibroblasts contributes to tumor progression. Authors: Yamamura Y, Asai N, Enomoto A, Kato T, Mii S, Kondo Y, Ushida K, Niimi K, Tsunoda N, Nagino M, Ichihara S, Furukawa K, Maeda K, Murohara T, Takahashi M Cancer Res, 2015;75(5):813-23. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA. Authors: Ota, Haruko, Hikita, Takao, Sawada, Masato, Nishioka, Tomoki, Matsumoto, Mami, Komura, Masayuki, Ohno, Akihisa, Kamiya, Yukiyo, Miyamoto, Takuya, Asai, Naoya, Enomoto, Atsushi, Takahashi, Masahide, Kaibuchi, Kozo, Sobue, Kazuya, Sawamoto, Kazunobu Nat Commun, 2014;5(0):4532. Species: Mouse Sample Type: Cell Lysates Application: WB Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina. Authors: Ito T, Komeima K, Yasuma T, Enomoto A, Asai N, Asai M, Iwase S, Takahashi M, Terasaki H Am J Pathol, 2013;182(2):586-96. Species: Human Sample Type: whole cells Application: WB Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain. Authors: Ota H, Hikita T, Nishioka T, Matsumoto M, Ito J, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K Biochem Biophys Res Commun, 2013;442(1):16-21. Species: Mouse Sample Type: tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Girdin Asn1510-Leu1824 Accession # Q3V6T2
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Girdin (GIRDers of actINfilaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196 - 425 and 458 - 1358), and one phosphoinositide-binding domain (aa 1390 - 1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
运输条件	Blue Ice
存放说明	4℃
参考文献	Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway

参考图片

Detection of Human Girdin by Western Blot. Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.