| 货号 | AF5328-SP |
| 别名 | CD300 antigen like family member G; CD300 antigen-like family member G; CD300 molecule like family member g; CD300 molecule-like family member g; CD300g antigen; CD300LG; CLM9; CLM-9; CLM9Trem4; CMRF35-like molecule 9; Nepmucin; TREM4; TREM-4; Triggering receptor expressed on myeloid cells 4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD300g/Nepmucin is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 146894 (Human); 52685 (Mouse); 684984 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD300g/Nepmucin Leu19-Leu249 Accession # Q6UXG3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Nepmucin (A mucin not expressed in Peyers Patches; also CD300g, CLM9, and TREM4) is a 75-95 kDa, O-glycosylated member of the CD300 family of molecules. It is expressed on capillary endothelium, and appears to serve multiple functions. Via a mucin domain, it binds lymphocyte L-selectin, contributing to cell migration. It also binds immunoglobulins A and M, perhaps mediating transendothelial transport. Mature human nepmucin is a 314 amino acid (aa ) type I transmembrane glycoprotein. It contains one V-type Ig-like domain (aa 19-121), a mucin stalk (aa 137-237), and a cytoplasmic region (aa 269-332). There are at least two isoform variants of nepmucin. Each shows a deletion of aa 128-212, accompanied by either a 23 aa, or 12 aa substitution for aa 296-332. Over aa 19-249, human nepmucin shares 51% aa identity with mouse nepmucin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CD300g/Nepmucin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD300g/Nepmucin at approximately 75-95 kDa and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
