| 货号 | AF5315-SP |
| 别名 | EC 3.1.4.11; KIAA1964; MGC71172; Phosphoinositide phospholipase C-delta-3; phospholipase C, delta 3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3; Phospholipase C-delta-3; PLC delta3; PLCD3; PLC-delta-3 | 全称 | Phospholipase C delta 3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse PLC‑δ3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 113026 (Human); 72469 (Mouse); 287745 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PLC‑δ3 Lys148-Ala344 Accession # Q8N3E9 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PLC-δ3 (phospholipase C-delta 3) is an 105 kDa member of the δ-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-δ3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca++ release, and DAG, activating PKC. Human PLC-δ3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-δ3 is 93% aa identical to mouse PLC-δ3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse PLC‑δ3 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC‑δ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC‑δ3 at approximately 100‑110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
