| 货号 | AF5310-SP |
| 别名 | Heterotaxy 1; HTX1; HTXHTX1; Zic family member 3 (odd-paired homolog, Drosophila); Zinc finger protein 203; zinc finger protein ZIC 3; ZNF203; ZNF203Zinc finger protein of the cerebellum 3 | 全称 | Zic Family Member 3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse ZIC3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7547 (Human); 22773 (Mouse); 367944 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZIC3 His380-Val467 Accession # O60481 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N‑terminal poly-Ala (aa 46‑55) and poly-His (aa 87‑97) segments, followed by five C2H2-type Zn-fingers (aa 253‑410). Within the zinc finger region, there is an overlapping NES (aa 290‑349) and two NLSs (aa 367‑382 and 403‑410). ZIC3 reportedly binds to GLI-3. There is one potential splice form that shows a 49 aa substitution for aa 409‑467, and a second splice form that shows a three aa addition to the C‑terminus. Over aa 380‑467, human ZIC3 displays absolute/100% identity to mouse ZIC3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse ZIC3 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5310) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZIC3 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ZIC3 in A172 Human Cell Line. ZIC3 was detected in immersion fixed A172 human glioblastoma cell line using Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5310) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
