| 货号 | AF5309-SP |
| 别名 | FLJ20185; FLJ20185k; PP610; proline rich 5 (renal); protein observed with Rictor-1; Protor-1 | 全称 | Proline Rich 5 [Renal] |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human PRR5 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55615 (Human); 109270 (Mouse); 315189 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PRR5 Tyr126-Ala259 Accession # P85299 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PRR5 (Pro-rich protein 5; also Protor-1) is a 42‑46 kDa member of the Protor family of proteins. It is widely expressed, and forms part of mTORC2, an enzyme complex that regulates Atk and PKC alpha. At present, it appears PRR5 promotes mTORC2 activity. Human PRR5 is 388 amino acids (aa) in length. It possesses two RICTOR-interaction sites (aa 10‑95 and 188‑218) and a C-terminal Pro-rich region. There are multiple splice forms of PRR5. Either individually, or in-common, there is an alternate start site at Met10 ( beta isoform) and Met96 ( gamma isoform), a deletion of aa 45‑71, 161‑388, and 236‑388, a 17 aa insertion after Gly108, and a six aa substitution for aa 1‑107. Over aa 26‑259 of the 41 kDa beta isoform (NP_056181), there is 95% aa identity between human and mouse PRR5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PRR5 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
