| 货号 | AF5297-SP |
| 别名 | CD39 antigen-like 4; CD39L4; CD39L4NTPDase 5; CD39-like 4; EC 3.6.1; EC 3.6.1.42; EC 3.6.1.6; ectonucleoside triphosphate diphosphohydrolase 5; ENTPD5; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; MGC163357; MGC163359; NTPDase 5; NTPDase-5; Nucleoside diphosphatase; Pcph proto-oncogene protein; PCPH; proto-oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5 | 全称 | Ecto-Nucleoside Triphosphate Diphosphohydrolase 5 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human CD39L4/ENTPD5 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 957 (Human); 12499 (Mouse); 314312 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5 Val21-His428 Accession # O75356 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CD39L4 (also ER-UDPase, PCPH and Ectonucleoside triphosphate diphosphohydrolase 5/eNTPDase5) is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation-dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa identity with mouse CD39L4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CD39L4/ENTPD5 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human CD39L4/ENTPD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
