| 货号 | AF5290-SP |
| 别名 | DAP kinase 3; DAP-like kinase; death-associated protein kinase 3; Dlk; EC 2.7.11; EC 2.7.11.1; ZIP kinase isoform; ZIP; ZIPK; ZIPKFLJ36473; ZIP-kinase | 全称 | Death-associated Protein Kinase 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human DAPK3 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1613 (Human); 13144 (Mouse); 64391 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DAPK3 Arg347-Arg454 Accession # O43293 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DAPK3 (Death-associated protein kinase 3; also known as ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes. In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic. DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation. Human DAPK3 is 454 amino acids (aa) in length and contains one protein kinase domain (aa 134‑275) and a C-terminal Leu-zipper motif (aa 427‑441) that mediates homo- and hetero-dimerization. There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313‑454. This form has both a nuclear and cytosolic localization. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human DAPK3/ZIPK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DAPK3/ZIPK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DAPK3/ZIPK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
