| 货号 | AF5288-SP |
| 别名 | CAS; CASMGC117283; Cellular apoptosis susceptibility protein; chromosome segregation 1 (yeast homolog)-like; Chromosome segregation 1-like protein; CSE1 chromosome segregation 1-like (yeast); CSE1; CSE1L; exp2; exportin-2; Importin-alpha re-exporter; MGC130037; XPO2; XPO2MGC130036 | 全称 | Chromosome segregation 1-like protein |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat CSE1L in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1434 (Human); 110750 (Mouse); 362273 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CSE1L Ala739-His933 Accession # P55060 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CSE1L (Chromosome segregation 1-like protein; also known as Exportin-2 (XPO2) and Cellular Apoptosis Susceptibility (CAS)) is a 110 kDa member of the XPO2/CSE1 family of proteins. It is expressed in divergent proliferating cell types such as colon and mammary epithelium, fibroblasts, hepatocytes, and spermatogonia. It participates in the export of importin-alpha from the nucleus, and regulates p53-sensitive gene transcription. Human CSE1L is 971 amino acids (aa) in length and contains an N-terminal importin domain (aa 29‑102) and a DNA-association region (aa 372‑385). There are multiple splice variants. There is a six aa substitution for aa 190‑971, separate deletions of aa 133‑341 and 257‑312, an alternate start site at Met235 with a deletion of aa 350‑517, and an alternate start site at Met100 with a 25 aa substitution for aa 674 - 971. Over aa 739‑933, human CSE1L shows 100% aa identity to mouse CSE1L. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human/Mouse/Rat CSE1L/CAS by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitts lymphoma cell line, A20 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CSE1L/CAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSE1L/CAS at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
