| 货号 | AF5265-SP |
| 别名 | C16orf77 | 全称 | Interleukin 34 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human IL-34 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse IL-34 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND50) is typically 0.035-0.175 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 146433 (Human); 76527 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL‑34 Asn21-Pro242 Accession # NP_689669 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL‑34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human IL‑34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265). The ND50 is typically 0.035‑0.175 µg/mL. | |
Detection of Human IL‑34 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IL‑34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑34 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
