Human ST2/IL-1 R4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human ST2/IL-1 R4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF523-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF523-SP
别名	DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2; T1
全称	Interleukin 33 Receptor
反应种属	Human
应用	Western Blot(0.2 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) ST2/IL-33 R is observed.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC). Blom, L and Poulsen, LK (2012) JI 189:4331. The Neutralization Dose (ND₅₀) is typically 0.1-0.6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Detection of soluble ST2 in human follicular fluid and luteinized granulosa cells. Authors: Southcombe J, Ledee N, Perrier dHauterive S, Turner K, Child T, Snider J, Redman C, Sargent I, Granne I PLoS ONE, 2013;8(9):e74385. Species: Human Sample Type: Cell Lysate Application: WB ST2 and IL-33 in pregnancy and pre-eclampsia. Authors: Granne I, Southcombe JH, Snider JV PLoS ONE, 2011;6(9):e24463. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Authors: Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y Gut, 2010;59(4):531-41. Species: Human Sample Type: Cell Lysates Application: WB A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H J. Allergy Clin. Immunol., 2008;121(6):1484-90. Species: Human Sample Type: Whole Cells Application: Neut Gene induction during differentiation of human pulmonary type II cells in vitro. Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL Am. J. Respir. Cell Mol. Biol., 2006;34(6):727-37. Species: Human Sample Type: Tissue Homogenates Application: WB IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Authors: Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA Immunity, 2005;23(5):479-90. Species: Human Sample Type: recombinant IL-33/ST-2 complex Application: WB Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK J Korean Med Sci, 0;26(9):1132-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R Lys19-Phe328 Accession # BAA02233
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33’s ability to signal through transmembrane ST2 (10, 13‑15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31. Tominaga, S.et al. (1992) Biochim. Biophys. Acta 1171:215. Li, H. et al. (2000) Genomics 67:284. Schmitz, J. et al. (2005) Immunity 23:479. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979. Brint, E.K. et al. (2004) Nat. Immunol. 5:373. Weinberg, E.O. et al. (2002) Circulation 106:2961. Sanada S. et al. (2007) J. Clin. Invest. 117:1538. Palmer, G. et al. (2008) Cytokine 42:358. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723. Miller, A.M. et al. (2008) J. Exp. Med. 205:339. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

参考图片

Detection of Human ST2/IL-33 R by Western Blot. Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-HumanST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ST2/IL-33 R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ST2/IL-33 R in KG‑1a Human Cell Line by Flow Cytometry.
KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

IFN-gamma Secretion Induced by
IL‑33 and Neutralization by Human ST2/IL-33 R Antibody. Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523). The ND₅₀ is typically 0.1-0.6 μg/mL.