| 货号 | AF5226-SP |
| 别名 | basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix family, member e41; BHLHB3; BHLHB3class E basic helix-loop-helix protein 41; BHLHE41; Class B basic helix-loop-helix protein 3; DEC2Enhancer-of-split and hairy-related protein 1; Differentially expressed in chondrocytes protein 2; SHARP-1; SHARP1hDEC2 | 全称 | Differentially expressed in chondrocytes protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human DEC2 direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DEC1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79365 (Human); 79362 (Mouse); 117095 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DEC2 Ala101-Gly287 Accession # Q9C0J9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human DEC2 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
