| 货号 | AF5208-SP |
| 别名 | Acylglycerol O-acyltransferase; adiponutrin; ADPN; ADPNdJ796I17.1; C22orf20; Calcium-independent phospholipase A2-epsilon; chromosome 22 open reading frame 20; EC 2.3.1.-; EC 2.7.7.56; EC 3.1.1.3; EC 4.2.3.4; FLJ22012; iPLA(2)epsilon; iPLA2-epsilon; patatin-like phospholipase domain containing 3; patatin-like phospholipase domain-containing protein 3; PNPLA3 | 全称 | Patanin-like Phospholipase Domain-containing Protein 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Adiponutrin/PNPLA3 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Adiponutrin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80339 (Human); 362972 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Adiponutrin/PNPLA3 Arg160-Arg349 Accession # Q9NST1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Adiponutrin/ADPN in Human Adipocytes. Adiponutrin/ADPN was detected in immersion fixed human adipocytes using 10 µg/mL Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Adiponutrin/ADPN by Western Blot. Western blot shows lysates of human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/ADPN at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
