| 货号 | AF5207-SP |
| 别名 | C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse CCR4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1233 (Human); 12773 (Mouse); 171054 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CCR4 Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284 Accession # P51680 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCR4 (C-C chemokine receptor 4) is a 42 kDa member of the GPCR #1 family of transmembrane proteins. In rodent, functional CCR4 is expressed on fully differentiated CD4+ Th2 cells, invariant CD4+ NKT cells, and neurons. It is the receptor for CCL17/TARC, CCL22/MDC and CKLF1, and responds to these ligands by initiating chemotaxis. Mouse CCR4 is a 7-transmembrane protein that is 360 amino acids (aa) in length. It contains a 39 aa N-terminal extracellular domain, and a 52 aa C-terminal cyoplasmic tail that shows a poly-Ser motif between aa 343-346. Over aa sequences 1-39, 99-111, 176-205 and 268-284 collectively, mouse CCR4 shares 85% and 93% aa identity with human and rat CCR4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse CCR4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and EL‑4 mouse lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 |
