| 货号 | AF5166-SP |
| 别名 | COE3; COE3OE-2; DKFZp667B0210; early B-cell factor 3O/E-2; EBF-3; OE-2; Olf-1/EBF-like 2; transcription factor COE3 | 全称 | Early B-cell Factor 3 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse EBF-3 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 253738 (Human); 13593 (Mouse); 361668 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse EBF-3 Ile399-Ser504 Accession # O08791-2 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EBF-3 (Early B cell Factor 3; also OE2 and COE3) is a 65 kDa (predicted) member of the COE family of transcription factors. It has limited expression, being identified principally in cerebellar Purkinje cells, olfactory neurons, and differentiating mantle layer neurons. Mouse EBF-3 is 596 amino acids (aa) in length it contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 371‑431, and a Pro/Ser-rich transactivation domain (aa 464‑555). EBF-3 either homodimerizes, or heterodimerizes with EBF-2 or -1. There are at least two potential splice variants, one that shows a nine aa deletion between aa 252 - 260, and a second that shows the same deletion coupled to a one aa substitution for aa 521‑557. Over aa 399‑504, mouse EBF-3 shows more than 99% aa identity to rat and human EBF-3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse EBF‑3 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Daudi human Burkitts lymphoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF‑3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
