| 货号 | AF5165-SP |
| 别名 | COE1; Collier, Olf and EBF transcription factor 1; early B-cell factor 1; Early B-cell factor; EBFFLJ41763; O/E-1; OE-1; OLF1; OLF1FLJ39389; olfactory neuronal transcription factor 1; transcription factor COE1 | 全称 | Early B-cell Factor 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse EBF-1 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1879 (Human); 13591 (Mouse); 116543 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jkappa and EBF1. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse EBF-1 Arg416-Ser520 Accession # Q07802 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EBF-1 (Early B cell Factor 1; also OLF1 and COE1) is a 65-70 kDa member of the COE family of transcription factors. Although expressed in adipocytes and neurons, it is best studied in B cells where IL-7 acts to promote EBF-1 in pre-proB cells, leading to proB stage development. Mouse EBF-1 is 591 amino acids (aa) in length. It contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 370‑430, and a Pro/Ser-rich transactivation domain (aa 462‑550). EBF-1 either homodimerizes, or heterodimerizes with EBF-2 and -3. There is an alternate start site at Met134, and an isoform that shows a one aa substitution for aa 252‑259. Over aa 416‑520, mouse EBF-1 shows absolute aa identity to the equivalent sequence in rat and human EBF-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human/Mouse EBF‑1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Daudi human Burkitts lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line, and L1.2 mouse pro‑B cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF‑1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
