| 货号 | AF5130-SP |
| 别名 | EC 2.7.11; EC 2.7.11.13; MGC57564; PKCC; PKCCSCA14; PKCG; PKC-gamma; PKCGprotein kinase C gamma type; PRKCG; protein kinase C, gamma; SCA14 | 全称 | Protein Kinase C gamma |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat PKC gamma in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5582 (Human); 18752 (Mouse); 24681 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKC gamma Arg621-Met697 Accession # P05129 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PKC gamma (protein kinase C-gamma) is a 78-80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++-activated and phospholipid-dependent neuronal enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC gamma is 697 amino acids (aa) in length. It contains two general regions: a lipid and Ca++-binding regulatory region (aa 35‑150 and 170‑260, respectively), and an ATP-binding catalytic region (aa 351‑614). PDK1-mediated phosphorylation of PKC gamma on Thr514 activates the enzyme. In addition, oxidative stress also mediates activation by inducing interchain disulfide-bond homodimerization. Over aa 621‑697, human PKC gamma is 99% and 95% aa identical to mouse and canine PKC gamma, respectively. PKC gamma is predominantly expressed in brain tissue. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PKC gamma by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5130) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
