| 货号 | AF5128-SP |
| 别名 | 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4; beta 4 (1-Phosphatidylinositol-4,5-BisphosphatePhosphodiesterase Beta 4)); EC 3.1.4.11; FLJ16169; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; phosphoinositide phospholipase C; phospholipase C, beta 4; PLCB4; triphosphoinositide phosphodiesterase | 全称 | Phospholipase C beta 4 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and, rat PLC-beta 4 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5332 (Human); 18798 (Mouse); 25031 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PLC-beta 4 Lys75-Gln250 Accession # Q15147 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PLC-beta 4 (phospholipase C-beta 4; also PLCretA) is a 130 kDa member of the beta -type PLC isozyme family. It is notably expressed in cerebellum and retina. PLC-beta 4 is associated with rhodopsin family GPCRs, where activation of Gq alpha subunits initiates PLC-beta 4 hydrolysis of PIP2. Human PLC-beta 4 is 1175 amino acids (aa) in length. It contains a catalytic X-box (aa 313‑463) and Y-box (aa 565‑681), followed by a calcium-binding C2 domain (aa 688‑786). There are multiple isoforms. One shows an alternate start site at Met114, a second contains a 40 aa substitution for the C-terminal 22 aa, a third shows a 14 aa substitution for the N-terminal 167 aa, and other forms demonstrate combinations of these alternative splice forms. Over aa 75‑250, human PLC-beta 4 is 97% aa identical to mouse PLC-beta 4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PLC-beta 4 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5128) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLC-beta 4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
