Human MMP-13 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human MMP-13 Affinity Purified Polyclonal Ab (25 UG)

货号： AF511-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF511-SP
别名	CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP-13
全称	Matrix Metalloproteinase 13
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human MMP-13 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-7, rhMMP-8, rhMMP-9, rhMMP-10 and rhMMP-12 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4322 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling. Authors: Yong HY, Kim IY, Kim JS, Moon A Int. J. Oncol., 2010;36(2):501-7. Species: Human Sample Type: Cell Culture Supernates Application: WB Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK FEBS Lett., 2007;581(9):1928-32. Species: Human Sample Type: Cell Culture Supernates Application: WB Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM J. Cell. Physiol., 2007;210(2):417-27. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation. Authors: Pillinger MH, Rosenthal PB, Tolani SN, Apsel B, Dinsell V, Greenberg J, Chan ES, Gomez PF, Abramson SB J. Immunol., 2003;171(11):6080-9. Species: Rabbit Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP-13 Leu20-Cys471 Accession # P45452
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
运输条件	Blue Ice
存放说明	4℃
参考文献	Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

参考图片

MMP‑13 in Human Ovarian Cancer Tissue. MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP‑13 in Human Breast. MMP‑13 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.