| 货号 | AF5109-SP |
| 别名 | HDLCQ9; Lipid transfer protein II; phospholipid transfer protein | 全称 | Phospholipid Transfer Protein |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human PLTP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PLTP is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5360 (Human); 18830 (Mouse); 296371 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1 Glu18-Val493 (Glu18Val) Accession # P55058 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Phospholipid transfer protein (PLTP; also known as lipid transfer protein II) is an 80 kDa, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily of molecules. It is synthesized as a precursor with a 17 amino acid (aa) signal sequence and a 476 aa mature chain. The mature chain contains two BPI/LBP/CETP domains (aa 21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP. PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung, adipose tissue, placenta, testis, brain, muscle, heart, liver. PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis. In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E. According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology. In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic. However, macrophage PLTP is shown to be atheroprotective. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of human PLTP by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
