| 货号 | AF5105-SP |
| 别名 | Nerve injury-induced protein 1; nerve injury-induced protein-1; NIN1; NINJ1; ninjurin 1; NINJURIN; ninjurin-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Ninjurin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Ninjurin-2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4814 (Human); 18081 (Mouse); 25338 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Ninjurin-1 Asp2-Val81 Accession # Q92982 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Ninjurin-1 (nerve injury-induced protein 1) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by Schwann cells, neurons and hepatocytes and participates in intercellular homophilic binding during nerve regeneration. Human Ninjurin-1 is 152 amino acids in length. It has an unusual membrane orientation. There is an 80 amino acid (aa) N-terminal extracellular domain (ECD) (aa 1-80), followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 142-152). Homophilic binding is divalent-cation dependent and occurs between Pro26 and Asn37. Over aa 1-80, human Ninjurin-1 shares 84% aa sequence identity with mouse Ninjurin-1. Human Ninjurin-1 shares only 50% aa sequence identity with human Ninjurin-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Ninjurin‑1 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Ninjurin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin‑1 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Ninjurin‑1 in Human Liver. Ninjurin‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Ninjurin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5105) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
