| 货号 | AF5085-SP |
| 别名 | complexin 2; Complexin II; complexin-2; CPLX2; CPX II; CPX2; CPX-2; CPX-II; DKFZp547D155; Hfb1; MGC138492; Synaphin 1; synaphin 1,921-L; synaphin-1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat Complexin-2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10814 (Human); 12890 (Mouse); 116657 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Complexin-2 Met1-Lys134 Accession # Q6PUV4 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Complexin-2 (CPX II; also known as synaphin-1) is a 19 kDa, cytosolic protein that belongs to the complexin/synaphin family of molecules. It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A. Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28‑84) that incorporates part of a SNARE complex interaction sequence (aa 41‑97). There is one potential splice variant that shows a premature truncation after Glu114. Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Complexin‑2 by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Complexin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Complexin‑2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Complexin‑2 in SH‑SY5Y Human Neuroblastoma Cells. SH‑SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Complexin-2 was detected using a Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
