| 货号 | AF5069-SP |
| 别名 | HUMORFA01; IQ motif containing GTPase activating protein 1; KIAA0051ras GTPase-activating-like protein IQGAP1; p195; p195RasGAP-like with IQ motifs; SAR1 | 全称 | IQ Motif Containing GTPase Activating Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human IQGAP1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8826 (Human); 544963 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IQGAP1 Lys505-Ser717 Accession # P46940 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IQGAP1 (IQ motif with Ras GAP-related domain 1) is a 195 kDa member of the IQGAP family of proteins. It serves as an effector protein for Rho family members, and regulates actin polymerization. Human IQGAP1 is 1657 amino acids (aa) in length. It contains one actin-binding CH domain (aa 45‑159), a Pro-binding WWP region (aa 685‑711), four consecutive calmodulin-binding IQ motifs (aa 745‑864), and a noncatalytic Ras-GAP domain (aa 1004‑1237). Amino acids 763‑863 mediate homodimerization. There are at least three IQGAP1 splice variants. One shows a two Lys substitution for aa 270‑1657, a second shows a ten Lys substitution for aa 299‑1657, and a third shows a three Lys substitution for aa 941‑1657. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human IQGAP1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC‑5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5069) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
