| 货号 | AF5067-SP |
| 别名 | EVH1/Sprouty domain containing protein; FLJ33903; hSpred1; NFLS; spred-1; sprouty-related, EVH1 domain containing 1; sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation | 全称 | Sprouty-related with an EVH1 Domain Containing-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human SPRED1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 161742 (Human); 114715 (Mouse); 296072 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis-1. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SPRED1 Arg118-Lys324 Accession # Q7Z699 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6‑123), a central KBD domain that binds to the SCFR (aa 233‑285), and a C-terminal Cys‑rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334‑442). There is one splice variant that shows a nine Lys substitution for aa 269‑444. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human SPRED1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human SPRED1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5067) followed by HRP-conjugated Anti‑Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED1 at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
