| 货号 | AF5066-SP |
| 别名 | EC 2.7.11; MAP3K10; MEKK10; mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 kinase; MLK2MKN28 derived nonreceptor_type serine/threonine kinase; MST; MSTEC 2.7.11.25; Protein kinase MST | 全称 | Mixed Lineage Kinase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human MLK2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4294 (Human); 269881 (Mouse); 308463 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MLK2 His345-Arg495 Accession # Q02779 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MLK2 (mixed lineage kinase 2; also MST and MAP3K10) is a member of the STE Ser/Thr kinase family of enzymes. It is expressed in neurons, lymphoid tissue, and skeletal muscle, where it participates in apoptosis. Human MLK2 is 954 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 23‑76), followed by a kinase domain that likely acts to phosphorylate Ser/Thr (aa 99‑355), two Leu-zipper segments that mediate homodimerization (aa 384‑419) and a C-terminal Ser/Thr/Pro-rich region that undergoes phosphorylation at multiple sites (aa 497‑954). There is one splice variant that shows an in-frame 16 aa substitution for the 16 aa between aa 465‑480. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MLK2 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK2 at approximately 104 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
