| 货号 | AF5064-SP |
| 别名 | KIAA0345-like 13; PCDHA1; PCDH-ALPHA1; PCDH-alpha-1; protocadherin alpha 1; protocadherin alpha-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Protocadherin alpha 1 (PCDH alpha 1) in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56147 (Human); 116731 (Mouse); 393085 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Protocadherin alpha 1 Gln30-Pro689 Accession # Q9Y5I3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protocadherin alpha 1 (PCDH alpha 1) is a group A member of the Protocadherin family of molecules. It is expressed in synaptic membranes of neurons, forms obligate Ca++ independent complexes with various PCDH gamma family members and demonstrates heterophilic binding activity towards beta 1 integrins. Human PCDH alpha 1 is 921 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 668 aa extracellular domain (ECD) plus a 232 aa cytoplasmic region. Both regions contribute to PCDH-gamma binding. There are six cadherin domains in the ECD. ADAM10 cleavage generates an 80‑90 kDa soluble fragment and a soluble splice form exists that is missing aa 535‑798. Over aa 30‑689, human PCDH alpha 1 shares 83% aa identity with mouse PCDH alpha 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Protocadherin alpha 1 in Mouse Brain. Protocadherin alpha 1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Sheep Anti-Human Protocadherin alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. | |
Detection of Human Protocadherin alpha 1 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin alpha 1 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Protocadherin alpha 1 in Mouse Brain. Protocadherin alpha 1 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Human Protocadherin alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
