| 货号 | AF5060-SP |
| 别名 | Activating enhancer-binding protein 2-epsilon; adaptor-related protein complex 2, epsilon subunit; AP2E; AP2-epsilon; MGC49007; TFAP2E; transcription factor AP-2 epsilon (activating enhancer binding protein 2epsilon); transcription factor AP-2-epsilon | 全称 | Activating Enhancer-binding Protein epsilon |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2δ, and rhAP‑2 gamma is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 339488 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AP-2 epsilon Ala123-Val217 Accession # Q6VUC0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human AP‑2 epsilon by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP‑2 epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP‑2 epsilon at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
