| 货号 | AF5038-SP |
| 别名 | FLJ36302; human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1; T-cell lymphoma invasion and metastasis 1; T-lymphoma invasion and metastasis-inducing protein 1 | 全称 | T-cell Lymphoma Invasion and Metastasis 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse TIAM1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7074 (Human); 21844 (Mouse); 304109 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TIAM1 Met1-Ala378 Accession # Q13009 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TIAM1 (T lymphoma invasion and metastasis) is an approximately 200 kDa member of the TIAM family of guanine-nucleotide exchange factors. It is expressed in multiple cell types, including endothelial cells, neurons, Schwann cells and almost all tumors. TIAM1 stimulates the activity of RHO-like GTPases and thus connects extracellular signals to the cytoskeleton. Human TIAM1 is 1591 amino acids (aa) in length. It contains a myristoylation site at Gly2, plus two pleckstrin homology domains (aa 434 - 549 and 1261 - 1397), a Ras-binding region (aa 765 - 832) and a RhoGEF/DH domain (aa 1041 - 1232). Over aa 1 - 378, human TIAM1 shows 94% aa identity to mouse TIAM1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse TIAM1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HCT‑116 human colorectal carcinoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
