| 货号 | AF5014-SP |
| 别名 | BSAS; homeo box A1; homeobox A1; Homeobox protein Hox-1F; homeobox protein Hox-A1; Hox 1.6-like protein; HOX A1 homeodomain protein; HOX1; HOX1F; HOX1Fhomeobox 1F; lab-like protein; MGC45232 | 全称 | Homeobox A1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human HOXA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) HOXB1, rhHOXB4, and rhHOXA9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3198 (Human); 15394 (Mouse); 25607 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human HOXA1 Met1-Asp118 Accession # P49639 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | HOXA1 (Homeobox A1; also Hox-1F) is a 35 kDa member of the Antp homeobox family of transcription factors. It is expressed in epithelium and is upregulated by E‑cadherin signaling, whereupon it suppresses apoptosis and promotes anchorage dependent growth. Human HOXA1 is 335 amino acids (aa) in length. It contains two poly-His sequences (aa 65-74 and 142-146), a PBX binding segment (aa 204-209) and a DNA binding homeobox domain (aa 229-288). There are multiple splice variants. There is a potential alternate start site at Met6 that may be paired with a nine aa substitution for the C‑terminal 117 aa, or a 19 aa substitution for the C‑terminal 217 amino acids. Over aa 1-118, human HOXA1 shares 92% and 90% aa identity with mouse and canine HOXA1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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HOXA1 in NTera-2 Human Cell Line. HOXA1 was detected in immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human HOXA1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HOXA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
