| 货号 | AF4996-SP |
| 别名 | DNA repair protein RAD50; EC 3.6; EC 3.6.1.15; EC 3.6.3; EC 3.6.3.27; hRad50; NBSLD; RAD50 (S. cerevisiae) homolog; RAD50 homolog (S. cerevisiae); RAD502; RAD50-2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Rad50 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10111 (Human); 19360 (Mouse); 64012 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Histone deacetylase inhibitor induces DNA damage, which normal but not transformed cells can repair. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Rad50 Leu518-Leu881 Accession # Q92878 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes. It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex. This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells. Human Rad50 is 1312 amino acids (aa) in length. It has an apparent ATP binding site (aa 36‑43) plus a coiled‑coil region (aa 228‑598) followed by a “zinc-hook” domain (aa 635‑734) that mediates homodimerization. There are multiple splice variants. An alternate start site exists at Met140, there is a single Lys substitution for aa 723‑1312, and three Lys substitute for aa 555‑1312. Over aa 518‑881, human Rad50 is 96% aa identical to mouse Rad50. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Rad50 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Rad50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad50 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
