| 货号 | AF4995-SP |
| 别名 | BAG; Brain Tumor Associated Protein LRRC4; Brain Tumor-Associated Protein BAG; Leucine Rich Repeat Containing 4; Leucine-Rich Repeat-Containing 4; Leucine-Rich Repeat-Containing Protein 4; NAG14; Nasopharyngeal Carcinoma-Associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2 | 全称 | Leucine-rich Repeat Containing 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LRRC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human LRRC3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64101 (Human); 192198 (Mouse); 641521 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human LRRC4 Ala39-Lys527 Accession # Q9HBW1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LRRC4 (Leu-rich repeat/LRR-containing protein 4; also brain tumor-associated protein) is a 55 kDa (predicted) member of the Leu-rich protein family. It is expressed on neurons and astrocytes, and antagonizes the signaling pathways for cytokines and growth factors. It is reduced in expression in tumors. Human LRRC4 is a type I transmembrane protein that is 653 amino acids (aa) in length. It contains a 489 aa extracellular region (aa 39-527), and a 105 aa cytoplasmic domain. The extracellular region shows nine LRRs (aa 74-288), a C2 type Ig-like domain (aa 354-440), and a Thr rich segment (aa 455-526). Over aa 39-527, human LRRC4 is 98% aa identical to both mouse and canine LRRC4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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LRRC4 in Human Brain. LRRC4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Sheep Anti-Human LRRC4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4995) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
